Journal: Materials (Basel, Switzerland)

Article Title: Bactericidal Activity of Graphene Oxide Tests for Selected Microorganisms.

doi: 10.3390/ma16114199

Figure Lengend Snippet: Figure 1. Increase in GO bactericidal level dependent on incubation time: (a) E. coli, (b) S. mutans, (c) S. aureus, (d) E. faecalis.

Article Snippet: Individual bacterial strains were cultured in species-optimal media: E. coli, LB medium (BTL, Lodz, Poland) [43]; S. mutans, MSB (Difco, Franklin Lakes, NJ, USA) [44]; S. aureus, BHI medium (Difco, Franklin Lakes, NJ, USA) [45]; and E. faecalis, BHI medium (Difco, Franklin Lakes, NJ, USA) [46].

Techniques: Incubation

Journal: Microbiology

Article Title: Degradation of SsrA-tagged proteins in streptococci

doi: 10.1099/mic.0.000048

Figure Lengend Snippet: S. mutans strains used in this study

Article Snippet: The abundance of cellular enolase was chosen as an internal control and was probed by an anti -S. mutans enolase rabbit polyclonal antibody (Genscript; I. Biswas, unpublished).

Techniques:

Journal: Microbiology

Article Title: Degradation of SsrA-tagged proteins in streptococci

doi: 10.1099/mic.0.000048

Figure Lengend Snippet: ClpXP degrades SsrA-tagged GFP in S. mutans. (a) Western blot analysis of GFPssrA and ClpX in the WT or clpX mutant transformed with an empty vector pIB190 (mock), clpX (pIBJ74, ClpX) or clpX mutant (pIBJ75, ClpXEGW). The blot was probed with anti-GFP antibody for GFP detection or anti-His antibody for ClpX detection. (b) Western blot analysis of GFP with or without different C-terminal tags in the WT S. mutans strain. The expression of enolase served as an internal control [detected using anti-enolase (Eno) antibody]. Protein expression from the plasmid is denoted by (p).

Article Snippet: The abundance of cellular enolase was chosen as an internal control and was probed by an anti -S. mutans enolase rabbit polyclonal antibody (Genscript; I. Biswas, unpublished).

Techniques: Western Blot, Mutagenesis, Transformation Assay, Plasmid Preparation, Expressing, Control

Journal: Microbiology

Article Title: Degradation of SsrA-tagged proteins in streptococci

doi: 10.1099/mic.0.000048

Figure Lengend Snippet: S. mutans ClpP is unable to degrade SsrA- or AVAA-tagged GFP in vitro. (a) In vitro degradation of GFP, GFPssrA or GFPavaa by (a) ClpXP or (b) ClpP was monitored by measuring green fluorescence in the reaction mixture. The relative value of the initial GFP fluorescence was set as 1.

Article Snippet: The abundance of cellular enolase was chosen as an internal control and was probed by an anti -S. mutans enolase rabbit polyclonal antibody (Genscript; I. Biswas, unpublished).

Techniques: In Vitro, Fluorescence

Journal: Microbiology

Article Title: Degradation of SsrA-tagged proteins in streptococci

doi: 10.1099/mic.0.000048

Figure Lengend Snippet: ClpE and ClpC contribute to the degradation of AVAA-tagged GFP in S. mutans. (a) Western blot analysis of GFPavaa in the WT, clpP mutant, clpX mutant, ΔclpXΔclpE double mutant and ΔclpXΔclpC double mutant. (b) Growth kinetics of the ΔclpP mutant, ΔclpX mutant and ΔclpXΔclpCΔclpE triple mutant are similar in the antibiotic-free medium. (c) Western blot analysis of GFPavaa degradation. Protein samples from the bacterial cells at exponential phase (E), late exponential phase (LE) or stationary phase (S) as indicated by arrows in (b). (d) Complementation of ClpE in the ΔclpXΔclpCΔclpE mutant. Western blot of GFPavaa in the ΔclpX mutant or ΔclpXΔclpCΔclpE mutant transformed with the vector pGhost9tr (mock) or pIBJ96 (ClpE). The presence of ClpE in the mutants and the complemented strains was probed by an anti-ClpE antibody. (e) Complementation of ClpC in the ΔclpXΔclpCΔclpE mutant. Western blot of GFPavaa in the ΔclpX or ΔclpXΔclpCΔclpE mutant transformed with the vector pGhost9tr (mock) and triple mutant transformed with pIBJ97 (ClpC). Endogenous enolase was used for normalization in all the Western blot analyses.

Article Snippet: The abundance of cellular enolase was chosen as an internal control and was probed by an anti -S. mutans enolase rabbit polyclonal antibody (Genscript; I. Biswas, unpublished).

Techniques: Western Blot, Mutagenesis, Transformation Assay, Plasmid Preparation

Journal: Microbiology

Article Title: Degradation of SsrA-tagged proteins in streptococci

doi: 10.1099/mic.0.000048

Figure Lengend Snippet: ClpCP- and ClpEP-mediated degradation is induced by heat stress. (a) Western blot analysis of GFPavaa in the ΔclpXΔclpC and ΔclpXΔclpCΔctsR mutants. Cultures at both exponential phase (E) and late exponential phase (LE) were investigated. (b) Kinetics of SsrA degradation during heat stress. Total proteins were extracted from S. mutans cells grown either at 37 or 44 °C at the indicated time points t. (c) Level of GFPavaa in cell extracts was determined by Western blot analysis using an anti-GFP antibody. Enolase was used as an internal control.

Article Snippet: The abundance of cellular enolase was chosen as an internal control and was probed by an anti -S. mutans enolase rabbit polyclonal antibody (Genscript; I. Biswas, unpublished).

Techniques: Western Blot, Control

Journal: Marine Drugs

Article Title: Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium

doi: 10.3390/md16020051

Figure Lengend Snippet: Biofilm inhibitory rates with different concentrations of dextranase.

Article Snippet: Cadex had inhibitory effects on S. mutans biofilm formation.

Techniques: Concentration Assay

Journal: Marine Drugs

Article Title: Purification and Characterization of a Biofilm-Degradable Dextranase from a Marine Bacterium

doi: 10.3390/md16020051

Figure Lengend Snippet: Electron microscopy of S. mutans biofilm formed on glass coverslips in the presence and absence of dextranase at different periods: ( Group a ) blank control, note the equal volume of cell-free pure water was added to replace dextranase; ( Group b ) biofilm subjected to 40 U/mL Penicillium dextranase ; and ( Group c ) biofilm subjected to 35 U/mL Cadex.

Article Snippet: Cadex had inhibitory effects on S. mutans biofilm formation.

Techniques: Electron Microscopy, Control

Journal: Viruses

Article Title: Genomic Analysis of a Novel Phage Infecting the Turkey Pathogen Escherichia coli APEC O78 and Its Endolysin Activity

doi: 10.3390/v13061034

Figure Lengend Snippet: The host range of the phage vB_EcoM_APEC and the lytic spectrum of endolysin LysO78.

Article Snippet: CCTCC AB99010 , Streptococcus mutans , − , −.

Techniques:

Journal: International Journal of Applied and Basic Medical Research

Article Title: Quantitative Polymerase Chain Reaction Analysis of Cariogenic Streptococcus mutans in Saliva of Oral and Laryngeal Cancer Patients Undergoing Radiotherapy: A Clinical Study

doi: 10.4103/ijabmr.IJABMR_151_19

Figure Lengend Snippet: The equipment required for the study. (a) Cylindrical graduated tubes and funnel. (b) pH strips. (c) Micro tubes, micropipettes and Eppendorf tubes. (d) Eppendorf tubes containing saliva and tris ethylenediaminetetraacetic acid buffer. (e) Realplex software integrated with polymerase chain reaction machine. (f) SYBR green fluorescence chart produced in real-time polymerase chain reaction for amount of Streptococcus mutans

Article Snippet: A mixture containing extracted DNA, SYBR green master mix, primers of S. mutans (Bioserve India Pvt Ltd., Hyderabad, India) and water were mixed and rotated at a slow speed.

Techniques: Software, Polymerase Chain Reaction, SYBR Green Assay, Fluorescence, Produced, Real-time Polymerase Chain Reaction

Journal: International Journal of Applied and Basic Medical Research

Article Title: Quantitative Polymerase Chain Reaction Analysis of Cariogenic Streptococcus mutans in Saliva of Oral and Laryngeal Cancer Patients Undergoing Radiotherapy: A Clinical Study

doi: 10.4103/ijabmr.IJABMR_151_19

Figure Lengend Snippet: (a) Average value of quantification of Streptococcus mutans before and after radiotherapy in Group I and II. (b) Mean value of salivary pH before and after radiotherapy in Group I and II. (c) Mean value of salivary flow rate before and after radiotherapy in Group I and II

Article Snippet: A mixture containing extracted DNA, SYBR green master mix, primers of S. mutans (Bioserve India Pvt Ltd., Hyderabad, India) and water were mixed and rotated at a slow speed.

Techniques:

Journal: International Journal of Applied and Basic Medical Research

Article Title: Quantitative Polymerase Chain Reaction Analysis of Cariogenic Streptococcus mutans in Saliva of Oral and Laryngeal Cancer Patients Undergoing Radiotherapy: A Clinical Study

doi: 10.4103/ijabmr.IJABMR_151_19

Figure Lengend Snippet: Comparison of streptococcus mutans , salivary pH and salivary flow rate pre- and post-radiotherapy in patients of oral cancer (Group-I)

Article Snippet: A mixture containing extracted DNA, SYBR green master mix, primers of S. mutans (Bioserve India Pvt Ltd., Hyderabad, India) and water were mixed and rotated at a slow speed.

Techniques:

Journal: International Journal of Applied and Basic Medical Research

Article Title: Quantitative Polymerase Chain Reaction Analysis of Cariogenic Streptococcus mutans in Saliva of Oral and Laryngeal Cancer Patients Undergoing Radiotherapy: A Clinical Study

doi: 10.4103/ijabmr.IJABMR_151_19

Figure Lengend Snippet: Comparison of streptococcus mutans , salivary pH and salivary flow rate pre- and post-radiotherapy in patients of laryngeal cancer (Group-II)

Article Snippet: A mixture containing extracted DNA, SYBR green master mix, primers of S. mutans (Bioserve India Pvt Ltd., Hyderabad, India) and water were mixed and rotated at a slow speed.

Techniques:

Journal: International Journal of Applied and Basic Medical Research

Article Title: Quantitative Polymerase Chain Reaction Analysis of Cariogenic Streptococcus mutans in Saliva of Oral and Laryngeal Cancer Patients Undergoing Radiotherapy: A Clinical Study

doi: 10.4103/ijabmr.IJABMR_151_19

Figure Lengend Snippet: Comparison of streptococcus mutans , salivary pH and salivary flow rate pre- and post-radiotherapy between Group I and Group II

Article Snippet: A mixture containing extracted DNA, SYBR green master mix, primers of S. mutans (Bioserve India Pvt Ltd., Hyderabad, India) and water were mixed and rotated at a slow speed.

Techniques:

Journal: Dental Press Journal of Orthodontics

Article Title: Effect of 8% arginine toothpaste on Streptococcus mutans in patients undergoing fixed orthodontic treatment: randomized controlled trial

doi: 10.1590/2177-6709.27.3.e2220322.oar

Figure Lengend Snippet: Thermal procedure and primer sequences of S. mutans used in the PCR and real-time PCR.

Article Snippet: Primer sequences of S. mutans (synthesized by Metabion, Munich, Germany) used in the PCR and real-time PCR are shown in .

Techniques: Sequencing

Journal: Dental Press Journal of Orthodontics

Article Title: Effect of 8% arginine toothpaste on Streptococcus mutans in patients undergoing fixed orthodontic treatment: randomized controlled trial

doi: 10.1590/2177-6709.27.3.e2220322.oar

Figure Lengend Snippet: Relative number of S. mutans in both study groups at different time intervals ( T0: At the beginning of the study, T1: After 30 days ).

Article Snippet: Primer sequences of S. mutans (synthesized by Metabion, Munich, Germany) used in the PCR and real-time PCR are shown in .

Techniques: